First report of Groundnut bud necrosis virus infecting anthurium ( Anthurium andreanum ) in India

Plant regeneration of Anthurium andreanum cv Rubrun

To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 μ M BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 μ M BA and 0.05 μ M NAA. On average, 3.6 shoots per explant were obtained. Four weeks o...

Differentiated Microelement Content in Anthurium (anthurium Cultorum Birdsey) Leaves*

The main objective of the study was to determine the nutritional status in terms of the content of microelements, such as iron, manganese, zinc, copper and boron in several standard cultivars of anthurium (Anthurium cultorum Birdsey) called Baron, Choco, Midori, Pistache, President and Tropical. The plants were grown in expanded clay (ř 8-18 mm) using drip fertigation with standard nutrient for...

Immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus.

In vitro gene expression strategy was used for the production of polyclonal antiserum to the nucleocapsid protein (NP) of Groundnut bud necrosis virus (GBNV). The GBNV NP gene from cowpea isolate was cloned into 6x His-tagged UA cloning vector and expressed in Escherichia coli [M15] cells. The fusion protein was detected in insoluble fraction and was purified by using Ni-NTA agarose resin. The ...

Bud necrosis of groundnut (Arachis hypogaea) in India caused by tomato spotted wilt virus*

Chlorotic ringspots or chlorotic leaf specking, terminal bud necrosis, axillary shoot proliferation and severe stunting of groundnut (Arachis hypogaea) were shown to be caused by tomato spotted wilt virus (TSWV). Cowpea (Vigna unguiculata cv. C-152) was found to be a good assay host. TSWV remained infective in buffered sap of groundnut at a dilution of 10-2’5, after storage for 4 h at room temp...

Micropropagation of Anthurium spp